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Animal perfusion and tissue processing for imaging After the ischaemia/reperfusion procedure, rats were given an overdose of pentobarbital sodium (200 mg/kg, i.v.) and transcardially perfused with 200 ml saline followed by 200 ml 4% paraformaldehyde (PFA) for fixation and then by a solution of 5% gelatine (Sigma-Aldrich, G2625) containing 0.25% FITC-albumin conjugate (Sigma-Aldrich, A9771) for visualisation of the perfused coronary microvasculature
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