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The NGs were treated 20 min at 37 °C with 0.1–0.5 mg ml −1 collagenase Ia (Sigma), 0.4–0.6 mg ml −1 dispase II (Roche, Basel, Swiss), 15 µg ml −1 DNase II type IV (Sigma), and 0.75 mg ml −1 bovine serum albumin (Sigma) in HKRB supplemented with 5.6 glucose
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Surprisingly, both Gcg and Dpp-4 genes are also expressed in platypus and echidna venom (Fig
