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The cAMP concentration in lysate was determined using the Dual-luciferase reporter assay system from Promega according to the manufacturer’s instructions with an EnVision plate reader (PerkinElmer, Waltham, MA, USA)
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Intensive Behavioral Therapy (IBT): Medicare Part B covers IBT for obesity (face-to-face counseling sessions with a primary care provider in a primary care setting) at no cost to the beneficiary
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