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Real time PCR was performed using an ABI platform with a 20-μl total reaction volume that included 100–1000 ng of cDNA and each primer at 100–200 nm (supplemental Table S3) plus 10 μl of 2× SYBR Green Master Mix (Applied Biosystems)

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TRPV4 Is a Regulator of Adipose Oxidative Metabolism, Inflammation, and Energy Homeostasis