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Research Models Where Tirzepatide Has Been Studied Across the published literature, tirzepatide has been investigated in: ·Cell-based receptor binding and activation assays at GIPR, GLP-1R, and (as a selectivity control) the glucagon receptor ·Signal transduction assays measuring cAMP accumulation and β-arrestin recruitment ·Cryo-EM structural studies of ligand–receptor–G protein complexes ·Rodent models of glucose regulation and energy balance ·Human clinical trial programmes conducted with the licensed drug product (see below) A Note on the Clinical Literature Tirzepatide differs from most compounds covered in this series in one important respect: it has an extensive human clinical trial literature , because it is the active ingredient of an approved prescription medicine