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The substitution of Ala 8 with D-Ala resulted in less than a twofold effect on affinity and less than a 10-fold reduction in potency at hGLP1-R (Xiao et al ., 2001), while substitution by Ser resulted in a ninefold reduction in affinity at rGLP-1R, with no effect upon potency (Adelhorst et al ., 1994)
