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2.18 Fluorescence correlation spectroscopy (FCS) FCS was performed as previously described (Corriden et al ., 2014) on a Zeiss LSM 510NLO ConfoCor 3 microscope fitted with a 40X c-Apochromat 1.2 NA water-immersion objective

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He did this by splicing the fishs DNA and inserting this into bacteria to be cloned