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On the day of the assay, plates were removed from the incubator and each well was washed twice with 100 μl HBSS (Gibco, Life Technologies) and pre-incubated for 30 min at 37 °C with 85 μl HBSS per well
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Spironolactone Based on 7 publication(s) in Google Scholar Spironolactone is an aldosterone antagonist that acts on the aldosterone mineralocorticoid receptor ( IC 50 =24 nM) and androgen receptor ( IC 50 =77 nM), promotes podocyte autophagy and regulates pain
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