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CPA digestion of albumin To initiate in vitro enzymatic cleavage by CPA, unfused albumin (WT or QMP) or albumin fused to GLP-1, FIX 9 , or ACE2 13 (15 µM), in DPBS (pH 7.4) (Sigma-Aldrich), were incubated with 30 µg/mL of human pancreatic CPA (Elastin Products Company, Inc.) for 5 h at 37 °C on a shaker platform set to 450 RPM
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For instance, in both H9c2 cardiomyoblasts and HepG2 hepatocellular carcinoma cells, liraglutide reduces pyroptosis by upregulating SIRT1 expression, scavenging intracellular reactive oxygen species (ROS), and enhancing mitophagy (Yu et al., 2019)
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